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The Sony Sorting Chip 100um for SH800 and MA900 is a microfluidic chip designed for use with Sony's SH800 and MA900 cell sorters. It features 100um wide channels and is made of optically clear cyclic olefin polymer. The chip allows high speed sorting of particles and cells from 10-30um in diameter. It comes in a box containing 40 chips and is intended for research use only. The chips integrate with the cell sorter's fluidics system to enable users to isolate populations of interest.
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Uses a DNA-specific dye so there is no need to use RNase.The dye crosses membranes so there is no need to permeabilize or fix the cells.This also means you can use live cells.No wash needed simply add and read for fast easily automated workflow.PrincipleCells stained with the cell cycle dye will fluoresce in proportion to their DNA content. The cell cycle consists of four distinct phases: G0/G1 phase growth S phase synthesis G2 phase and M phase mitosis . Each phase can be identified based on the relative amounts of DNA resident in the cell with G0/G1 cells having the least amount of DNA and G2/M cells with the most. These relative differences in DNA content are easily detected by fluorescence intensity and can be utilized to establish quantitative cell cycle profiles.Multiplex with other iQue kits like Cell Membrane Integrity for richer content.
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Ability to encode cell and measure cell proliferation and using the violet laser.Multiplex with other MultiCyt kits like Cell Membrane Integrity or Qbeads for richer content.Dyes are safe and non-toxic to the cells.Covalently attaches to intracellular proteins enabling its long-term retention.PrincipleProliferation Use: The principle of dye dilution. Cell proliferation is detected and quantified based on the halving or “dilution” of the loaded dye after each round of cellular division. Through subsequent cell divisions daughter cells retain approximately half the fluorescent label of the parent cells.Encoding Use: Multiple cell populations are stained with different intensities of dye distinguish up to 4 cell populations Bright Mid Dim and Unstained . Target cells are stained with different concentrations of V/Blue encoding dye in order to discriminate the different cell populations.
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Ability to measure cell viability and using the violet laser.No wash protocol just add and read for fast easily automated workflow.Multiplex with other Intellicyt kits like Cell Cycle for richer content.PrincipleThe Cell Membrane Integrity dyes are fluorescent molecules that are cell impermeant. Healthy cells with intact cell membranes will exclude the dyes. Unhealthy cells with compromised membranes will allow entry of the dye into the intracellular space. Upon entry to the cell the dye localizes to the nucleus and binds to DNA by intercalation.Multiplex with other Intellicyt kits like Cell Cycle for richer content.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
Sterile, non-pyrogenic flow cytometry shutdown fluid for CYTOFLEX™ prevents salt crystal formation & biological growth in fluidics system during storage with 0.1% sodium azide preservative for CYTOFLEX™ flow cytometer maintenance & research use only.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More